Description: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: BRA-FB6
Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
Antibody Reactivity: MHC-II
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Tissue Antigens (1988) 32(1):6-11. (IP)
- Cancer Sci (2008) 99(8): 1633-1642. (ELISPOT)
- World J Gastroenterol (2003) 9(5): 1077-1081. (Flow, surface)
- Eur J Immunol (2009) 39: 1947-1955. (functional studies)
Entrez Gene ID: 3115
Expected AB Applications: ELISA (published for clone)/Flow, surface (published for clone)/Functional studies (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow (verified)/Flow, surface (published)/Functional studies (published)/ELISA (published)/IP (published)
Verified AB Applications: Flow (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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