Mouse Notch1 Pathway Reporter Kit (Notch Signaling Pathway)

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen for activators or inhibitors of the Notch signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the Notch pathway.

Background: The Notch signaling pathway controls cell fate decisions in vertebrate and invertebratetissues. Notch signaling is triggered through the binding of a transmembrane ligand,such as Delta like ligand 4 (DLL4) or jagged 1 (JAG1) to a Notch transmembranereceptor (Notch1, Notch2, Notch3, Notch4) on a neighboring cell. This results inproteolytic cleavage of the Notch receptor, releasing the constitutively active intracellulardomain of Notch (NICD). NICD translocates to the nucleus and associates withtranscription factors CSL (CBF1, RBPJ
, Suppressor of Hairless, Lag-1) and thecoactivator, Mastermind, to turn on transcription of Notch responsive genes.

Concentration: The Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each). The Notch1 Expression Vector and Negative Control Expression Vector are provided at 100 ng DNA/µl (250 µl each).

Description: The Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready expression vector for Notch1 with the entire extracellular domain deleted (Notch1DE). Inside the cells, the Notch1DE can be cleaved by -secretase and active Notch1 NICD is released to nucleus. The kit also contains a CSL (CBF1/RBP-J
) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized CSL responsive element upstream of a minimal promoter. The CSL (CBF1/RBP-J
) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.

Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Lu FM et al. (1996). Proc. Natl. Acad. Sci. USA 93(11): 5663-5667.
2. Kanungo et al (2008). J.Neurochem. 106 2236.Application Reference:
MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1 (2014)

• Artikelnummer: BPS60509
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60509
• Green Labware: Nein
• Verpackungseinheit: 500 rxns.
• Mengeneinheit: PAK