Description: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: 45M1
Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Antibody Reactivity: Mucin 5AC
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Biochem J (2002) 364, 191-200. (IHC, FFPE; ELISA)
- FEBS Journal 275 (2008): 481-489. (WB; epitope mapping)
- Cancer Immunol Immunother (2013) 62: 1011-1019. (Flow, surface)
Entrez Gene ID: 4586
Expected AB Applications: ELISA (published for clone)/Flow, surface (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/ELISA (published)/IHC, FFPE (verified)/WB (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Flow cytometry: 0.5-1 ug/million cells in 0.1 mL/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology formalin-fixed 1-2 ug/mL/Does not react with cow, others not known/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
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