Description: Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: 5.8A
Immunogen: Recombinant mouse MyoD1 protein
Antibody Reactivity: MyoD1
References: Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41. / Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. / Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11. /
Entrez Gene ID: 4654
Antibody Application Notes: For coating for ELISA, order Ab without BSA/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry/Only nuclear staining should be considered as evidence of skeletal muscle differentiation/Optimal dilution for a specific application should be determined by user
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