Target: Notch1
Conjugate: RPE
Product Type: Monoclonal
Clone Number: N253/32 (Formerly sold as S253-32)
Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
Swiss-Prot: Q01705
Purification: Protein G Purified
Storage Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration: 1 mg/ml
Specificity: Detects >270kDa. Does not cross-react with Notch 2 or Notch3.
Cellular Localization: Cell Membrane / Nucleus
Scientific Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
References: 1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719.2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.3. Lindsell C.E., et al. (1995) Cell 80: 909-917.4. Uyttendaele H., et al. (1996) Development 122: 2251-2259.5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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