Conjugate: ATTO 488
Product Type: Monoclonal
Clone Number: N408/79 (Formerly sold as S408-79)
Immunogen: Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Swiss-Prot: Q8CJH6
Purification: Protein G Purified
Storage Buffer: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration: 1 mg/ml
Specificity: Detects ~90kDa.
Cellular Localization: Cytoplasm / Nucleus
Tissue Specificity: Highly expressed in the brain, with lower expression found in endocrine tissue.
Scientific Background: NPAS4 (Neuronal PAS Domain Protein 4) is a brain-specific transcription factor that plays a pivotal role in regulating activity-dependent gene expression critical for synaptic plasticity, learning, and memory. As a member of the bHLH-PAS family, NPAS4 is rapidly induced by neuronal activity and orchestrates the formation and maintenance of inhibitory synapses, particularly in excitatory neurons. NPAS4 modulates the excitatory-inhibitory balance in neural circuits by promoting the expression of genes involved in GABAergic synapse development. It also activates the CNS midline enhancer and regulates drebrin, a cytoskeletal protein essential for dendritic spine morphology. Dysregulation of NPAS4 has been linked to neurodevelopmental and neuropsychiatric disorders, including schizophrenia, autism spectrum disorder, and cognitive impairment. In neurodegenerative disease research, NPAS4 is gaining attention for its neuroprotective roles in response to excitotoxic stress and its potential to modulate synaptic resilience. Its activity-dependent expression profile makes it a promising biomarker and therapeutic target for preserving synaptic integrity in aging and disease.
References: 1. Zhaohui Z., et al. (2014) Exp Ther Med. 7(4): 1045-1049. 2. Coutellier L., et al. (2012) Plos One. 7(9): e46604.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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