Clone Name: DO1
Light Chain: Kappa
Product Description: p53 antibody recognizes p53 protein, which has a predicted molecular weight of 44 kDa but is typically detected by western blot at 53 kDa. p53, known widely as a tumor suppressor, plays critical roles in various cellular processes including cell cycle regulation, DNA repair, and apoptosis. p53 is mutated in many human cancers.
Application Note: WB: 1:500-1:10000. IHC-P: 1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 44
Positive Control: 293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)
Form: Liquid
Buffer (with preservative): PBS, 20% Glycerol, no Preservative.
Concentration: 0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Uniprot ID: P04637
Antigen Species: Human
Immunogen: Recombinant human p53 expressed in E. coli.
Purification: Protein A purified
Conjugation: Unconjugated
Full Name: tumor protein p53
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