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Encompassing Amino Acids: 2-393(end)
Description: Recombinant human p53(tumor protein p53), full length, encompassing amino acids 2-393(end). In this construct, the N-terminal end of the protein is fused to GST via a short peptide linker placed between GST consisting of an optimal TEV protease target sequence that allows cleavage of GST (TEV: tobacco etch virus cysteine protease >BPS Bioscience #50308). The construct also contains mutation of interest (Y220C). The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol
Genbank: NM_000546
Host Cell Line: Sf9 cells
Purity: ≥90%
Tags: N-terminal GST-tag
Uniprot: P04637
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)