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Encompassing Amino Acids: Full Length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction isconducted using BPS PARP Assay Kit(Catalog Number 80551) at roomtemperature for 1 hour.
Description: Human PARP1 (GenBankAccession No. NM_001618), full lengthwith N-terminal FLAG-Avi-tag™, MW = 117kDa, expressed in a Baculovirus infectedSf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 0.2mM TCEP, 80 µg/ml FLAG peptide
Genbank: NM_001618
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-Avi-tag™
Uniprot: P09874
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Aguilar-Quesada R, et al., Curr Med Chem. 2007;14(11):1179-87.
2. Pacher, P. and Szabo, C. Cardiovasc Drug Rev. 2007 Fall;25(3):235-60.