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PARPtrap™ Combo Assay Kit for PARP1 and PARP2
PARPtrap™ Combo Assay Kit for PARP1 and PARP2
Artikelnummer
BPS78317
Verpackungseinheit
384 rxns.
Hersteller
BPS Bioscience
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Applications: Great for screening small molecules that enhance PARP1/2 trapping on DNA in drug discovery and HTS applications, and for determination of compound IC50.
Allows direct comparison of drug effects on PARP1 and PARP2.
Background: PARP1 and PARP2 enzymes are known to bind damaged DNA through their DNA-binding domains. Binding to DNA activates these enzymes and in the presence of NAD+, PARP1 and PARP2 ribosylate themself (autoribosylation), leading to their dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, these PARPs remain bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment.
Contraindications: The PARPtrap™ Combo Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO aqueous solution and using 2.5 µl per well.
Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
Storage Stability: This assay kit will perform optimally for up to 1 year from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Murai, J. et al. Molecular Cancer Therapeutics 2014. 13: 433-4432. Murai, J. al. Cancer Research 2012. 72: 5588-55993. Zandarashvili, L. et al. Science 2020. 368(6486): 30-31
Applications: Great for screening small molecules that enhance PARP1/2 trapping on DNA in drug discovery and HTS applications, and for determination of compound IC50.
Allows direct comparison of drug effects on PARP1 and PARP2.
Background: PARP1 and PARP2 enzymes are known to bind damaged DNA through their DNA-binding domains. Binding to DNA activates these enzymes and in the presence of NAD+, PARP1 and PARP2 ribosylate themself (autoribosylation), leading to their dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, these PARPs remain bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment.
Contraindications: The PARPtrap™ Combo Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO aqueous solution and using 2.5 µl per well.
Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
Storage Stability: This assay kit will perform optimally for up to 1 year from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Murai, J. et al. Molecular Cancer Therapeutics 2014. 13: 433-4432. Murai, J. al. Cancer Research 2012. 72: 5588-55993. Zandarashvili, L. et al. Science 2020. 368(6486): 30-31
| Artikelnummer | BPS78317 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 78317 |
| Green Labware | Nein |
| Verpackungseinheit | 384 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
