Shelf life (days): 730.0
Assay Range: 117.2-15,000 pg/ml
Sensitivity: 80% B/B0: 350 pg/ml
Cross Reactivity: Prostaglandin D2 100%; Prostaglandin F2α 92.4%; Prostaglandin J2 21.6%; Prostaglandin E2 2.86%; Thromboxane B2 2.54%; 11β-Prostaglandin F2α 1.99%; 8-iso Prostaglandin F2α 1.90%; Prostaglandin A2 0.72%; 12(S)-HHTrE 0.16%; 6-keto Prostaglandin F1α 0.05%; 13,14-dihydro-15-keto Prostaglandin D2 0.02%; Arachidonic Acid <0.01%; Leukotriene D4 <0.01%; tetranor-PGDM <0.01%; tetranor-PGEM <0.01%; tetranor-PGFM <0.01%; tetranor-PGJM <0.01%
Formula Weight: 0.0
Notes: Prostaglandin D2 (PGD2) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF2α and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman’s PGD2 Express ELISA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions. This ELISA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an assay range of approximately 117.2-15,000 pg/ml and a sensitvity (80% B/B0) of approximately 350 pg/ml.
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