Research Areas: Others
Uniprot: P04483
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Form: Liquid or Lyophilized powder
Tag Info: Tag-Free
Purity: Greater than 85% as determined by SDS-PAGE.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Molecular Weight: 23.4 kDa
Gene Names: tetR
Organism: Escherichia coli
Source: E.coli
Expression Region: 1-207aa
Protein Length: Full Length
Target Protein Sequence: MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
Endotoxin: Not test.
Relevance: TetR is the repressor of the tetracycline resistance elent; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
Reference: Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant.Postle K., Nguyen T.T., Bertrand K.P.Nucleic Acids Res. 12:4849-4863(1984)
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