Protein Tag: N-His
Uniprot: P46013
Accession: P46013
Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly.Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface.Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.Binds DNA, with a preference for supercoiled DNA and AT-rich DNA.Does not contribute to the internal structure of mitotic chromosomes.May play a role in chromatin organization.It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Bio Acitivity: Not validated for activity
Sequence: Lys1001-Pro1112
Purity: > 85% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 12.4 kDa
Observed MW: 13 kDa
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