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Protein Tag: C-His
Uniprot: P05164
Accession: P05164
Background: Myeloperoxidase (MPO) is a heme-containingenzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein thatcatalyzes the hydrogen peroxide-dependentconversion of chloride, bromide, and iodide to multiple reactive species. Post-translationalprocessing of MPO involvesthe insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linkeddimer composed of a60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bondbetween the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion ofthe signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and theformation of 3-chlorotyrosineand dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levelsof NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilicgranules. Upon cellular activation, it is deposited into pathogen-containingphagosomes.
Bio Acitivity: Not validated for activity
Sequence: Ala49-Ser745
Purity: > 95% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 1.0 EU/mg of the protein as determined by the LAL method.
Calculated MW: 80.3 kDa
Observed MW: 85-95 kDa
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