Target Synonym: OTB;OTU;OTUB;Ubiquitin Thioesterase OTUB;Deubiquitinating Enzyme OTUB;Ubiquitin-Specific-Processing Protease OTUB;OTU Comain-Containing Ubiquitin Aldehyde-Binding Protein;C14orf;Otubain;Deubiquitinating Enzyme OTUB2;OTU Comain-Containing Ubiquitin Aldehyde-Binding Protein 2;Otubain-2;Ubiquitin Thioesterase OTUB2;Ubiquitin-Specific-Processing Protease OTUB2;C14orf137;OTB2;OTU2;OTUB2;FLJ21916;MGC3102;OTU domain;OTU domain containing ubiquitin aldehyde binding protein 2;OTU domain-containing ubiquitin aldehyde-binding protein 2;Otubain 2;ubiquitin aldehyde binding 2;Ubiquitin specific processing protease OTUB2;Ubiquitin specific protease otubain 2
Background: Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
Immunogen: Recombinant Human OTUB2 Protein
Buffer: 0.2 μm filtered solution in PBS
Dilution: ICC/IF 1:20-1:100
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