Target Synonym: H2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;gamma H2A.X;γH2AX
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Immunogen: A synthetic peptide corresponding to residues around Ser139 of Human Phospho-Histone H2A.X
Buffer: 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5
Dilution: WB 1:2000-1:20000;IHC-P 1:1000-1:5000
Calculated MW: 15 kDa
Observed MW: 15 kDa
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