Target Synonym: MGC;Tnfsf;TNF-a;Cachectin;DIF;ICD1;ICD2;Membrane Form;MGC124630;N-terminal fragment;NTF;RATTNF;Soluble Form;TNF-alpha;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2;Tnf;Tnfa;Tnfsf2
Background: TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Immunogen: Recombinant Rat TNF-alpha Protein
Buffer: 0.2 μm filtered solution in PBS
Dilution: ICC/IF 1:20-1:100
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