Description: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1, 709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: HSn 7D2
Immunogen: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
Antibody Reactivity: CD169/Sialoadhesin/SIGLEC1
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Cancer Sci (2013) 104(9): 1237-1244. (WB; cell ELISA; IHC, FFPE)
- Rheumatology (2014) 53(2): 250-259. (Flow, surface; functional studies)
Entrez Gene ID: 6614
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/IHC (FFPE) (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IHC, FFPE (published)/WB (published)
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