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SIK (SNF1LK), His-tag Recombinant
SIK (SNF1LK), His-tag Recombinant
Artikelnummer
BPS40274
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-303
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate SIK with 0.2 mg/ml AMARA peptide substrate and 50 µM [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.
Description: Recombinant human SIK (GenBank Accession No. NM_173354), a.a. 1-303, with N-terminal His-tag, MW = 36 kDa, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Genbank: NM_173354
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P57059
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Takemori, H. Et al: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 2007; 274 (13): 3202-9.
2.Sjostrom, M.; SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. 104: 16922-16927, 2007.
Encompassing Amino Acids: 1-303
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate SIK with 0.2 mg/ml AMARA peptide substrate and 50 µM [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.
Description: Recombinant human SIK (GenBank Accession No. NM_173354), a.a. 1-303, with N-terminal His-tag, MW = 36 kDa, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Genbank: NM_173354
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P57059
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Takemori, H. Et al: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 2007; 274 (13): 3202-9.
2.Sjostrom, M.; SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. 104: 16922-16927, 2007.
| Artikelnummer | BPS40274 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40274 |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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