SOX10 (Melanoma Marker) (rSOX10/1074), CF740 conjugate, 0.1mg/mL

SOX10 (Melanoma Marker) (rSOX10/1074), CF740 conjugate, 0.1mg/mL
Artikelnummer
BTMBNC742312-500
Verpackungseinheit
500 uL
Hersteller
Biotium

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Description: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rSOX10/1074

Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Antibody Reactivity: SOX10

Entrez Gene ID: 6663

Z-Antibody Applications: IHC, FFPE (verified)/WB (verified)

Verified AB Applications: IHC (FFPE) (verified)/WB (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
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Artikelnummer BTMBNC742312-500
Hersteller Biotium
Hersteller Artikelnummer BNC742312-500
Verpackungseinheit 500 uL
Mengeneinheit STK
Reaktivität Human, Mouse (Murine)
Klonalität Recombinant
Methode Western Blotting, Immunohistochemistry
Isotyp IgG1 kappa
Wirt Mouse
Konjugat Conjugated, CF740
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