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Encompassing Amino Acids: full length
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 20 µM Sphingosine and 20 µM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Assay was done in Kinase buffer (40 mM Tris, pH 7.4, 20 mM MgCl2+, 0.1 mg/ml BSA) containing 20 µM Sphingosine and 20 µM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Description: Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.
Genbank: NM_001142601.1
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9NYA1-1
Unit Definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004).
2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).Application Reference:Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)
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