Target: Tau
Conjugate: ATTO 390
Product Type: Monoclonal
Clone Number: 1D5
Immunogen: Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils
Swiss-Prot: P10636
Purification: Protein G Purified
Storage Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
Concentration: 1mg/mL
Specificity: Detects Multiple Bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).
Cellular Localization: Cytoplasm / Axolemma / Axolemma Plasma Membrane / Axon / Cell Body / Cell membrane / Cytoplasmic Ribonucleoprotein Granule / Cytoplasmic Side / Cytoskeleton / Cytosol / Dendrite / Growth cone / Microtubule / Microtubule Associated Complex / Neurofibrillary Tangle / Neuronal Cell Body / Nuclear Periphery / Nuclear Speck / Nucleus / Peripheral membrane protein / Plasma Membrane / Tubulin Complex
Tissue Specificity: Expressed in neurons.
Scientific Background: Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).
References: 1. www.alz.org/alzheimers-dementia/facts-figures2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6):667-77.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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