Description: This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: TA99
Immunogen: SK-MEL-23 cells
Antibody Reactivity: TRP1
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Invest Dermatol (1985) 85:169-174. (IP; IHC, frozen)
- PNAS USA (1987) 84, 4200-4204. (in vivo imaging melanoma)
- J Exp Med (1995) 182: 1609-1614. (functional studies)
- J Cell Biol (2015) 209(4): 563-577. (Flow, surface; IF)
- J Cell Mol Med (2017) 21(4): 802-815. (IHC, FFPE)
Entrez Gene ID: 7306
Expected AB Applications: Flow, surface (published for clone)/IHC (FFPE) (published for clone)/IHC (frozen) (published for clone)/IF (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, surface (published)/IF (published)/IHC, FFPE (published)/IHC, frozen (published)/IP (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology (frozen only) 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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