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Encompassing Amino Acids: 2-981(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay done according to USP15 Inhibitor Screening Assay Kit (BPS Bioscience #82940) with USP15, His-tag, FLAG-tag Recombinant titrated at various amounts.
Background: USP15 is a deubiquitinase that associates with the COP9 (constitutive photomorphogenesis 9) signalosome (CSN) complex. It regulates the NF-kappaB pathway by deubiquitinating IkappaBalpha2. USP15 acts as a key regulator of TGF-beta receptor signaling pathway and is thought to promote deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. UPS15 is linked to immune and inflammatory roles, where it can regulate the transcription of pro-inflammatory cytokines and type I interferons. It also plays a role in the TEM (tumor microenvironment). More research will shed light on the role of USP15 as a cancer therapy target.
Description: Recombinant human USP15 (ubiquitin carboxyl-terminal hydrolase 15), full length encompassing amino acids 2-981(end). This construct contains an N-terminal His-tag (6xHis) and a C-terminal FLAG-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable FLAG peptide, and 20% glycerol
Genbank: NM_001252078.2
Host Cell Line: HEK293
Purity: ≥75%
Storage Stability: >6 months at –80°C.
Tags: FLAG, His
Target: USP15
Uniprot: Q9Y4E8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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