Target: Versican
Conjugate: RPE
Product Type: Monoclonal
Clone Number: N351/23 (Formerly sold as S351-23)
Immunogen: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Swiss-Prot: Q62059
Purification: Protein G Purified
Storage Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration: 1 mg/ml
Specificity: Detects >350kDa.
Cellular Localization: Extracellular Space / Extracellular Matrix
Scientific Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.
References: 1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998.2. Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013.3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639.4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623.5. Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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