Products from BPS Bioscience require a minimum order value above 400€
Application: Activation and expansion of Vγ9Vδ2 T cell from freshly isolated or frozen PBMCs, for downstream applications in CAR (chimeric antigen receptor) γδ T-cell development, such as cytotoxicity assays and flow cytometry.
Background: T lymphocytes are composed of two subpopulations: αβ T-cells and γδ T-cells. They are distinguished by the expression of either an αβ TCR or a γδ TCR, respectively. αβ T-cells are the predominant subset of T cells in peripheral blood and recognize antigens presented by MHC (major histocompatibility complex) molecules. γδ T cells are less abundant and recognize antigens independently of MHC presentation. While both αβ T cells and γδ T cells contribute to cell cytotoxicity through distinct mechanisms to target and eliminate infected or abnormal cells, γδ T cells have a lower risk of causing GvHD (Graft-versus-Host Disease) when injected into humans and have demonstrated cytotoxicity against a wide range of tumor types. γδ TCRs are cell type-specific, with Vγ9Vδ2 being the predominant γδ T cell type in human peripheral blood. Vγ9Vδ2 T cells are involved mostly in immune responses to pathogens and long-term modulation of inflammation, and can recognize non-peptide phospho- antigens, alkylamines and synthetic amino-bisphosphonates. Vγ9Vδ2 T cells are being studied for the treatment of solid tumors and hematological disorders and are becoming a highly promising cancer therapy. Further studies on how best to utilize Vγ9Vδ2 T cells, and methods to enhance their presence, will open new therapeutic avenues for cancer and infections.
Description: Vγ9Vδ2 T Cell Expansion Kit is suitable for the ex vivo culture and expansion of human Vγ9Vδ2 T cells. γδ T cells are low abundance in PBMCs (< 2%) and therefore they need to be successfully activated and expanded to obtain adequate number of cells for γδ T-cell based immunotherapy related studies. This kit contains media and reagents necessary to drive the robust activation and expansion of the Vγ9Vδ2 T cells subpopulation from PBMCs. To improve the purity of the expanded cells, αβ T cells and B cells are depleted from the expansion culture using an antibody cocktail. This kit is provided with enough reagents and materials for the activation and expansion of Vγ9Vδ2 T cells from a starter population of 10 x 107 PBMCs.
Storage Stability: This assay kit will perform optimally for up to 6 months from the date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Allison T. and Garboczi D., 2002 Molecular Immunology 38 (14): 1051-1061.
Sawaisorn P., et al., 2024 Scientific Reports 14: 1291.
Deutsch
English
