Target: VGLUT1
Conjugate: RPE
Product Type: Monoclonal
Clone Number: N28/9 (Formerly sold as S28-9)
Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Swiss-Prot: Q62634
Purification: Protein G Purified
Storage Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration: 1 mg/ml
Specificity: Detects ~52kDa. No cross-reactivity against VGlut2.
Cellular Localization: Cytoplasmic Vesicle / Secretory Vesicle / Synaptic Vesicle Membrane / Membrane / Cell Junction / Synapse
Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
References: 1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163.2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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