Description: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. , 2004 ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse)
Conjugate: Purified, BSA-free
Concentration: 1 mg/mL
Storage buffer: PBS, no BSA, no azide
Clone: 2A2
Immunogen: Recombinant fragment (around aa 418-436) of human WIPI2 protein (exact sequence is proprietary)
Antibody Reactivity: WIPI2
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Autophagy (2010) 6(4):506-522. (IF)
- Brain (2019) 142(5): 1242-1254. (IF, IP, WB)
Entrez Gene ID: 26100
Expected AB Applications: IF (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: IF (published)/IP (published)/WB (published)
Antibody Application Notes: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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