WWP1 Intrachain TR-FRET Assay Kit

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Background: Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) is a HECT-type E3 Ub ligase belonging to the NEDD4 family of E3 ligases. WWP1 interacts with and ubiquitinates many substrates, including transcription factor ΔNp63 as well as phosphatase and tensin homolog (PTEN), and regulates the degradation of sodium channels and membrane receptors. Genetic mutations or regulatory defects involving WWP1 are associated with neurological disorders and cancer. Aberrant expression of WWP1 in gastric, prostate, and breast cancer, for instance, is an area of high interest, and therefore, WWP1 represents an excellent candidate for targeting in multiple cancer types.

Contraindications: The WWP1 intrachain TR-FRET Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 5% DMSO solution in buffer and using 4 µl per well.

Description: The WWP1 intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin (donor) as well as a Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on WWP1, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetic analyses of polyubiquitination.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 

Supplied As: The WWP1 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure WWP1 auto-ubiquitination activity in a homogeneous 384 reaction format.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78554
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78554
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK