eGFP/Firefly Luciferase JeKo-1 Cell Line

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Applications:

• Use as target cells in CAR-T or NK co-culture killing assays.
• In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging

Background: The JeKo-1 mantle cell lymphoma (MCL) cell line was isolated from the peripheral blood mononuclear cells of a 78-year-old female with a large cell variant of MCL showing leukemic conversion. The JeKo-1 cells are highly tumorigenic in SCID mice and have applications in immunology research. These cells overexpress cyclin D1, Bcl-2 (B-cell lymphoma 2), c-Myc and Rb proteins. A Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The presence of eGFP and luciferase allow for easy assay readouts, making this cell line a convenient choice.

Description: The eGFP/Firefly Luciferase JeKo-1 Cell Line is a JeKo-1 cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter, generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741), which is a SIN0 self-inactivating lentivirus).

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ~1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS82147
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82147
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK