Products from BPS Bioscience require a minimum order value above 400€
Application: Use as a negative control when testing CD38-directed strategies in A549 cells. Study the phenotype derived from CD38 knockout.
Background: CD38 (Cluster of Differentiation 38, Cyclic ADP-Ribose Hydrolase 1, or ADPRC1) is a glycoprotein and ectoenzyme playing an important role in regulating intracellular calcium. CD38 is a highly attractive target antigen for immunotherapy because it is highly expressed on multiple myeloma (MM) cells, and at relatively low levels on normal lymphoid and myeloid cells. Expression of CD38 has also been associated with HIV infection, leukemia, and type II diabetes mellitus (T2D). In 2015, the FDA approved daratumumab (Darzalex), a breakthrough therapy monoclonal antibody targeting CD38, for the treatment of MM. CD38 has become an interesting target for the development of CAR (chimeric receptor antigen)-T cells, which are engineered by retroviral transduction to express a fully human CD38-specific CAR. In addition, the level of CD38 upregulation in lung cancer patients correlates with patient survival, with its enzymatic activity leading to increased cell migration, proliferation and tumor progression. Future development and studies will open new therapeutic avenues for CD38-related diseases, such as lung cancer.
Description: Firefly Luciferase CD38 Knockout A549 Cell Line is an A549 human lung cancer cell line constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter, where CD38 (Cluster of Differentiation 38) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human CD38. This cell line was generated by using Firefly Luciferase Lentivirus (#78740-H) on CD38 Knockout A549 Cell Line (#82953). This cell line has been validated by genomic sequencing, flow cytometry and luciferase activity.
Host Cell Line: A549 is a human lung alveolar basal carcinoma cell line. Adherent epithelial cells.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (#79796)
Target: CD38
Uniprot: P28907
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: Cui Q., et al., 2021 J Hematol Oncol. 14(1):82. Gao L., et al., 2021 Cell Death Dis. 12(7):680.
Deutsch
English
