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Application: Screen therapeutic antibodies, ADCs (antibody drug conjugates), or CAR (chimeric antigen receptor) constructs targeting GUCY2C.
Background: Human GUCY2C (Guanylate cyclase 2C) is a membrane-bound receptor primarily expressed in intestinal epithelial cells and, to a lesser extent, in other tissues such as the liver and lung. GUCY2C plays a key role in regulating intestinal fluid and electrolyte homeostasis by catalyzing the conversion of GTP to cyclic GMP (cGMP) upon activation by endogenous ligands such as guanylin and uroguanylin, or by bacterial heat-stable enterotoxins. It is a crucial component of intestinal barrier function and fluid secretion. Dysregulation of GUCY2C signaling has been implicated in gastrointestinal disorders and colorectal cancer. Due to its restricted normal expression and overexpression in certain malignancies, GUCY2C has also emerged as a promising biomarker and therapeutic target for colorectal cancer and other gastrointestinal tumors.
Description: GUCY2C HEK293 Cell Line is a HEK293 cell line expressing GUCY2C (guanylate cyclase 2C; NM_004963.4) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lipophilic transfection followed by hygromycin selection and limited dilution. Individual clones were screened for GUCY2C expression levels by flow cytometry, and a low expressing clone and a high expression clone were selected to generate these cell lines. This cell line has been validated by flow cytometry. Purchase of this cell line is for research purposes only; commercial use requires a separate license.
Genbank: NM_004963.4
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: GUCY2C
Uniprot: P25092
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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