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Application: Screen antibodies targeting ITGA4/ITGB7.
Background: Integrins are heterodimeric glycoprotein receptors involved in the regulation of cell adhesion and motility. They are comprised of both an alpha and beta subunit and are classified based upon the ligand motif to which they bind. The Integrin α4β7 (ITGA4/ITGB7) is widely expressed on lymphocytes where it acts as a T cell homing receptor that directs migration to the gut. This process is triggered by the binding of ITGA4/ITGB7 with its ligand, mucosal addressin-cell adhesion molecule 1 (MADCAM-1) which is expressed at high levels in inflamed intestinal tissue. In irritable bowel disease (IBD), ITGA4/ITGB7 is upregulated on activated T cells where it plays an important role in driving chronic inflammation. Integrin blocking therapies have been explored to decrease immune cell homing and subsequent tissue inflammation in irritable bowel diseases including ulcerative colitis (UC) and Crohn’s disease (CD), dry eye disease, and multiple sclerosis. Since the ligand for ITGA4/ITGB7, MADCAM-1, is primarily expressed in the intestines, anti-ITGA4/ITGB7 therapies such as Vedolizumab show a gut restricted anti-inflammatory effect without systemic immunosuppression, making them an appealing therapeutic avenue for IBD.
Description: The ITGA4/ITGB7 HEK293 Cell Line is a HEK293 cell line expressing the human ITGA4 (integrin α4, also known as CD49d) (NM_000885.6) and ITGB7 (integrin β7) (NM_000889.3) separated by a P2A self-cleaving peptide. This cell line was generated by lentiviral transduction followed by puromycin selection and limiting dilution. Individual clones were screened based on ITGA4/ITGB7 expression by flow cytometry to obtain this ITGA4/ITGB7 expressing cell line. This cell line was validated by flow cytometry.
Genbank: NM_000885.6, NM_000889.3
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ≥1 x 106 cells in 1 ml of Cell Freezing Medium (#79796)
Target: ITGA4, ITGB7
Uniprot: P13612, P26010
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: DeNucci C., et al., 2010 J Immunol. 184(5):2458-67.Dotan I., et al., 2020 Med Res Rev. 40(1):245-262.Feagan B., et al., 2005 N Engl J Med. 352(24):2499-507.Slack R., et al., 2022 Nat Rev Drug Discov. 21(1):60-78.Soler D., et al., 2009 J. Pharmacol. Exp. Ther 330(3): 864–875Yu Y., et al., 2012 J Cell Biol. 196(1):131-46.
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