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Application: Use as target cells in CAR-T or CAR-NK co-culture killing assays.In vitro and in vivo bioluminescence imaging.
Background: HepG2 cell line is a human hepatoma cell line derived from a hepatocellular carcinoma. HepG2 cells have an epithelial-like morphology and a high proliferation rate. This cell line is commonly used in drug metabolism, hepatotoxicity, and liver disease research due to its ability to perform many liver-specific functions in vitro. Studies have shown that glypican-3 (GPC3) is significantly up-regulated in HepG2 cell line, making it an excellent cell line for studying the role of GPC3 in hepatocellular carcinoma and potentially develop targeted therapies. Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. It was first cloned from the North American Photinus pyralis and catalyzes the oxidation of D-luciferin, in the presence of ATP and magnesium, emitting yellow light. This reaction has a high quantum yield, and both luciferase and luciferin have low toxicity. These characteristics contributed to Firefly luciferase becoming a commonly used tool. The use of firefly luciferase as reporter allows for easy readouts.
Description: Firefly Luciferase HepG2 Cell Line is a liver cancer HepG2 cell line that expresses the firefly luciferase reporter driven by an EF1a promoter. This cell line has been generated by transduction with Firefly Luciferase Lentivirus (EF1A Promoter/ Hygromycin) (BPS Bioscience #78740-H).This cell line has been validated in co-culture assays.
Host Cell Line: HepG2, human hepatoma cell line, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ≥1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: GPC3
Unit Definition: Cell Line
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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