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Application: Study the impact of HER2 knockout.Use as a control for developing anti-HER2 CAR-T killing assays.
Background: HER2 (human epidermal growth factor receptor 2), also known as erbB-2 or CD340, is a tyrosine kinase receptor of the EFGR family of proteins. There is no known ligand, but it can form homodimers or heterodimers with other HER proteins. Once active, it activates the MAPK (mitogen-activated protein kinase) and PI3K (phosphatidylinositol-3 kinase) signaling pathways resulting in cell cycle progression and cell proliferation. HER2 over-expression is also known to occur in breast, ovarian, stomach, lung adenocarcinoma, aggressive forms of uterine cancer and gastric cancer. In 1990 the FDA approved the use of the monoclonal antibody trastuzumab in breast and stomach cancer. Other strategies to target HER2 that have been approved include ADCs (antibody-drug conjugate) and margetuximab (an anti-HER2 antibody that can alter the Fc-receptor affinity to CD16 and induce cytotoxicity). The use of small molecule tyrosine kinase inhibitors, alone or in combinatory therapy, has shown great promise in the treatment of HER2+ breast cancer (BC). However, resistance to treatment, for instance by mutations on HER2 or upregulation of other HER receptors, has been described. Neratinib, a pan-HER2 inhibitor, was approved in 2017 for early-stage BC, as adjuvant anti-HER2 therapy after trastuzumab treatment. However, side effects limit its use. The development of treatments targeting early-stage cancer, with minimal side effects and resistance development, will bring major benefits to HER2+ oncology patients.
Description: HER2 Knockout MCF7 Cell Line is an MCF7 cell line in which human HER2 (Receptor tyrosine-protein kinase ErbB-2) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding the CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human HER2. This cell line has been validated by sequencing and flow cytometry.
Host Cell Line: MCF7 human breast mammary gland cell line. Adherent epithelial cells.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: HER2
Uniprot: P04626
Unit Definition: Cell Line
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: Schlam I. and Swain S., 2021 npj Breast Cancer 7: 56.
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