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Application: Screen and characterize anti-IL-12 antibodies as well as IL-12 mimetics.
Background: IL-12, a heterodimeric complex composed of p40 and p35, is a proinflammatory cytokine produced by activated macrophages and B-lymphocytes. This cytokine plays a crucial role in differentiating CD4+ T-cell to TH1 cells which promote cell-mediated immunity to intracellular pathogens by secreting several cytokines including IFN-γ (interferon gamma). In humans, the involvement of IL-12 in autoimmune diseases like Crohn’s Disease (CD) and psoriasis has spurred significant efforts to develop reagents that antagonize its signaling pathway. However, recent research suggests that, paradoxically, stimulating IL-12 signaling could be a promising cancer immunotherapy strategy by stimulating both innate and adaptive immunity.
Description: IL-12 Responsive STAT4 Luciferase Reporter HEK293 Cell Line was engineered to express firefly luciferase under the control of interferon-γ-activated sequence of the interferon regulatory factor 1 (GAS-IRF1) promoter. It also expresses human STAT4 (signal transducer and activator of transcription 4) (NM_001243835.1), and the human IL-12 receptor complex (IL12Rβ1 NM_005535.3 and IL12Rβ2 NM_001559.3). With this cell line, IL-12 activity can be monitored by measuring luciferase activity.The functionality of the IL-12 Responsive STAT4 Luciferase Reporter HEK293 Cell Line was validated in dose-response assays using a recombinant human interleukin 12 (human IL-23; p35 + p40) and inhibition assays using an IL-12 neutralizing antibody (#102108). Figure 1. Illustration of the mechanism of action of the IL-12 Responsive STAT4 Luciferase Reporter HEK293 Cell Line. Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit our Immunotherapy Biochemical Screening Services.
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: IL-12, STAT4
Uniprot: A1Z2L5, Q14765
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: Benson J. M., et al., 2011 Nat. Biotechnology. 29(7): 615-624.
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