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Application: Studies involved PBMCs or isolated immune cells (such as T cells).
Background: Human peripheral blood mononuclear cells (PBMCs) include all the cells present in the peripheral blood that have a round nucleus. It is thus a mix of lymphocytes, such as T, B and NK cells, and monocytes. Most cells present in PBMCs are lymphocytes, with T cells being the main cell type. The proportion of each cell type present can be determined by using cell specific marker combinations, for instance NK cells are characterized as CD3-CD56+ cells. The use of these markers also allows for isolation of cell populations of interest. PBMCs are a critical and widely used cell source in the immunology, infectious disease, vaccine, cancer therapy and transplant immunology fields.
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.
Storage Stability: Store cells at -135°C or colder (liquid nitrogen). Thawed cells should be used immediately for downstream applications. As these are primary cells, we do not recommend maintaining these cells in culture.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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