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Application: Use in in vitro assays focused on immunological research, drug development, and cancer studies, including cytotoxicity assays, immunotherapy screening, drug screening, and immune activation assays.
Background: T lymphocytes are composed of two subpopulations: αβ T-cells and γδ T-cells. They are distinguished by the expression of either an αβ TCR or a γδ TCR, respectively. αβ T-cells are the predominant subset of T cells in peripheral blood and recognize antigens presented by MHC (major histocompatibility complex) molecules. γδ T cells are less abundant and recognize antigens independently of MHC presentation. While both αβ T cells and γδ T cells contribute to cell cytotoxicity through distinct mechanisms to target and eliminate infected or abnormal cells, γδ T cells have a lower risk of causing GvHD (Graft-versus-Host Disease) when injected into humans and have demonstrated cytotoxicity against a wide range of tumor types. γδ TCRs are cell type-specific, Vδ1 expressing T cells are found in mucosal and epithelial tissues and correspond to about 15% of the γδT cells present in PBMCs, with the % of γδ being only 5% of all the T cells. Vδ1 T cells are highly cytolytic, meaning they can effectively kill tumor cells. Engineering Vδ1 T cells to express chimeric antigen receptors (CARs) further enhances their antitumor activity and antigen specificity.
Description: Expanded Human Peripheral Blood Gamma Delta T Cells (Vδ1), Frozen, are primary Vδ1 T cells enriched and expanded from human PBMCs (Peripheral Blood Mononuclear Cells) using Membrane Bound IL-15 Based Growth-Arrested Feeder Cells (#82374), and cryopreserved. Expanded Human Peripheral Blood Gamma Delta T Cells (Vδ1), Frozen, are >90 % pure Vδ1 T cells, as measured by flow cytometry analysis. They can be used in cytotoxicity assays and other in vitro assays, after thawed in T Cell Medium (#78753), or further expanded if using feeder cells.
Mycoplasma Testing: The cells have been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of CryoStor® CS10 (Stemcell Technologies #100-1061)
Target: T Cell
Unit Definition: Primary Cells
Warnings: Avoid freeze/thaw cycles.Donors have been screened and determined negative for:Hepatitis B (anti-HBc EIA, HBsAg EIA)Hepatitis C (anti-HCV EIA)Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)Human T-Lymphotropic Virus (HTLV-I/II)HIV-1/HCV/HBVWest Nile VirusTrypanasoma cruziNote: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.
Biosafety Level: BSL-1
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