Cyclin E1 antibody (CCNE1/2460)

Clone Name: CCNE1/2460

Light Chain: Kappa

Application Note: WB: 1-2μg/ml. IHC-P: 1-2μg/ml for 30 min at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 47

Form: Liquid

Buffer (with preservative): 10mM PBS, 0.05% BSA, 0.05% Sodium azide.

Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Uniprot ID: P24864

Antigen Species: Human

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Purification: Protein A/G purified

Conjugation: Unconjugated

Full Name: cyclin E1