Her2 / ErbB2 antibody (ERBB2/2452)

Clone Name: ERBB2/2452

Light Chain: Kappa

Application Note: IHC-P: 1-2μg/ml for 30 min at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 138

Specificity/Sensitivity: Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain.

Form: Liquid

Buffer (with preservative): 10mM PBS, 0.05% BSA, 0.05% Sodium azide.

Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot ID: P04626

Antigen Species: Human

Immunogen: Recombinant human HER-2 protein fragment (around aa 311-462) (exact sequence is proprietary)

Purification: Protein A/G purified

Conjugation: Unconjugated

Full Name: erb-b2 receptor tyrosine kinase 2