Streptavidin (HRP)

Clone Name:

Product Description: Streptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Protein content: > 95%. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.

Form: Liquid

Buffer (with preservative): 10mM Phosphate, 200mM NaCl (pH7.5), 10mg/ml BSA, 0.05% ProClin 300.

Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)

Background: Streptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.

Conjugation: HRP