Primary Cell Optim. 96-well Kit (160RCT)

The Primary Cell Optimization 96-well Shuttle™ Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well Shuttle™ Add-On.
With the unique capability of the 96-well Shuttle™ Add-On to address individual wells of a 96-well Nucleocuvette™ Plate with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using one 96-well Nucleocuvette™ Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell Nucleofector™ Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette™ Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector™ Solutions and Nucleofector™ Programs.

Content:
•0.675 mL Nucleofector™ Solution P1
•0.675 mL Nucleofector™ Solution P2
•0.675 mL Nucleofector™ Solution P3
•0.675 mL Nucleofector™ Solution P4
•0.675 mL Nucleofector™ Solution P5
•4 x 0.15 mL Supplement 1