hPB CD4+ Naïve, 5M cells

Leukapheresed Naïve CD4+ T cells are negatively selected using a two step isolation process. CD4+ T Cells are first negatively selected from mononucleated cells using an indirect immunomagnetic CD4+ labeling system. CD45RO microbeads are then used to deplete the CD45RO+ population, leaving a highly enriched CD4+ CD45RA+ Naïve T Cell population. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI.