HUVECs cultured in EGM™-Plus Growth Media offer faster proliferation rates while maintaining the same high quality characterization as HUVECs in EGM™ MediaLonza's Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD), express CD31/105, von Williebrand Factor VIII, and are positive for acetylated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.