Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.
CloneticsTM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. CloneticsTM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.