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Encompassing Amino Acids: full length
Applications: Useful as a substrate in kinase assays.
Description: Human Akt2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Genbank: NM_001626
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P31751
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Alessi et al., Current Biology 8: 69, 1998.
2. Cohen et al., FEBS Lett. 410: 3, 1997.