Aurora A Protein Kinase, His-Tag (Sf9-derived) Recombinant

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Encompassing Amino Acids: 2-403

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM Aurora A substrate, and 4 µg/ml Aurora A kinase.

Description: Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.

Genbank: NM_003600

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: O14965

Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003).

• Artikelnummer: BPS40004
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40004
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect