DNA Polymerase β Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen molecules that inhibit polymerization activity of DNA Polymerase β in drug discovery and high throughput screening (HTS) applications.Determine compound IC₅₀.

Background: Polymerases are the enzymes responsible for synthesizing nucleic acids. DNA Polymerase β, also known as DNA polymerase subunit beta, POLB, belongs to the Family X of DNA polymerases and functions in base excision repair (BER). Its activity is crucial for DNA replication and drug resistance. Mitochondrial DNA is particularly prone to oxidative stress, and DNA Polymerase β is essential to maintain its stability.  DNA Polymerase β overexpression has been linked to cancer, while low levels of this enzyme can lead to hypersensitivity to alkylating agents and apoptosis. The inhibition of DNA synthesis can be used as a therapeutical approach for diseases in which cell division or energetic needs are uncontrolled, such as cancer. Inhibitors such as gemcitabine have proved efficacious in the treatment of hematological and solid tumors (including breast cancer and ovarian cancer), particularly when combined with cisplatin. The use of cisplatin results in severe side effects, so the use of DNA inhibitors can also benefit patients ʹ care by allowing lower doses of platinum drugs to be used.  Further studies into DNA polymerase inhibitors will bring new therapy options to cancer patients.

Description: The DNA Polymerase β Assay Kit is a fluorogenic assay designed to measure DNA Polymerase β activity for screening and profiling applications, using AccuGreen™ as detection reagent. The kit comes in a convenient 96-well format, with enough recombinant Polymerase β enzyme, DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.Note: AccuGreen™ High Sensitivity dsDNA Quantitation Kit is sold separately by Biotium.DNA Polymerase β uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecule (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can quantified by the addition of the fluorescent DNA-binding dye which allows to distinguish dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.

Storage Stability: This assay kit contains DNA and will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P06746

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Berdis A., 2017, Front. Mol. Biosci. 4:  doi.org/10.3389/fmolb.2017.00078

• Artikelnummer: BPS82099
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82099
• Green Labware: Nein
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK