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Applications: Useful for labeling cells expressing PD-L1 for flow cytometry, immunofluorescence microscopy, and live cell imaging.
Assay Conditions: For flow cytometry, we recommend incubating cells on ice for 30 minutes with 1-20 µg/ml of anti-human PD-L1 antibody, PE-labeled.
Description: R-Phycoerythrin-labeled anti-PD-L1 recombinant murine/human antibody recognizing the PD-1 binding region of human PD-L1. This antibody does not cross-react with mouse PD-L1, but does cross-react with monkey (M. fascicularis) PD-L1. It has not been tested with other species.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: At least 6 months at -80°C. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Uniprot: Q9NZQ7
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1.Freeman, G.J., et al., J. Exp. Med. 2000; 192(7): 1027-1034.2.Dong. H., et al., Nat. Med. 1999; 5(12):1365-1369.