Application Note: Human IgM Fc5µ antibody has been tested by ELISA and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Clone ID: 22G1.H8
Concentration Value: 1.0 mg/ml
ELISA Dilution: 1:1000 - 1:10000
Western Blot Dilution: 1:1000-1:2000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Human IgM Fc5µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
Background: Anti-Human IgM Fc5µ antibody generated in mice specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5µ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Low Endotoxin: No
Secondary Antibody Specificity: IgM Fc5µ
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